5TU9

Crystal structure of Staphylococcus epidermidis Aap G58-spacer-G513 (variant G5-spacer-consensus G5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.984
Spacegroup name	P 21 21 2
Unit cell lengths	43.560, 54.950, 184.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.396 - 1.900
R-factor	0.2183
Rwork	0.217
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.766
Data reduction software	XDS (March 1, 2015)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.10.1-2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.560	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.068	0.520
Number of reflections	43043
<I/σ(I)>	13.41	2.77
Completeness [%]	99.1	99
Redundancy	4.7	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M sodium acetate pH 5.0 0.2 M calcium chloride 25% PEG 6000