5TTR

LEU 55 PRO TRANSTHYRETIN CRYSTAL STRUCTURE

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1995-05
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	149.990, 78.740, 98.950
Unit cell angles	90.00, 100.50, 90.00

Refinement procedure

Resolution	8.000 - 2.700
R-factor	0.199 *
Rwork	0.199
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tta
RMSD bond length	0.019
RMSD bond angle	0.030
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.055	0.353
Number of reflections	31394
<I/σ(I)>	14.8	2.4
Completeness [%]	98.8	98.1
Redundancy	3.4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		drop contained 50:50 mixture of protein and reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	ammonium sulfate	45 (%sat)
3	1	reservoir	PEG400	7 (%)
4	1	reservoir	HEPES	100 (mM)