5TSY
Structure of the glycoside hydrolase domain of PelA variant E218A from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2016-05-31 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 65.421, 84.098, 47.179 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.359 - 1.900 |
| R-factor | 0.1861 |
| Rwork | 0.184 |
| R-free | 0.23080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tcb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.836 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.084 | 0.231 |
| Number of reflections | 21165 | |
| <I/σ(I)> | 17 | 5.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.6 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 0.1 M CHES pH 9.5 and 30% (w/v) PEG 3000 |
