5TSC

The crystal structure of Lpg2147 from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97915
Spacegroup name	P 1
Unit cell lengths	48.927, 68.373, 71.655
Unit cell angles	117.13, 98.77, 90.28

Refinement procedure

Resolution	36.025 - 2.008
R-factor	0.1753
Rwork	0.174
R-free	0.21040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5suj
RMSD bond length	0.005
RMSD bond angle	0.702
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1-2155_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.008	2.008
Rmerge	0.050	0.314
Number of reflections	52808
<I/σ(I)>	13.5	3.33
Completeness [%]	97.6	96.8
Redundancy	2.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	298	0.1 M bis-tris ph 5.5, 0.2 M MgCl2, 25% PEG3350