5TRL

Crystal structure of human GCN5 histone acetyltransferase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	77
Detector technology	CCD
Collection date	2015-07-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 3
Unit cell lengths	175.728, 175.728, 175.728
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.137 - 2.299
R-factor	0.1987
Rwork	0.197
R-free	0.22330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z4r
RMSD bond length	0.004
RMSD bond angle	0.776
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2152)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.146	0.046	0.998
Rmeas	0.145	0.047
Rpim	0.032	0.010	0.288
Total number of observations	1727504
Number of reflections	80269
<I/σ(I)>	5.5
Completeness [%]	100.0	100	99.9
Redundancy	21.5	21.1	12.8
CC(1/2)		1.000	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.4M Ammonium sulfate, 0.4M Lithium chloride