5TQA
Crystal Structure of DH270.6 (unliganded) from the DH270 Broadly Neutralizing N332-Glycan Dependent Lineage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.066, 73.718, 112.714 |
| Unit cell angles | 90.00, 107.18, 90.00 |
Refinement procedure
| Resolution | 48.360 - 2.723 |
| R-factor | 0.1884 |
| Rwork | 0.185 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tpp |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.912 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.360 | 50.000 | 2.780 |
| High resolution limit [Å] | 2.730 | 7.400 | 2.730 |
| Rmerge | 0.112 | 0.030 | 0.642 |
| Rmeas | 0.150 | 0.040 | 0.859 |
| Rpim | 0.099 | 0.027 | 0.565 |
| Total number of observations | 57894 | ||
| Number of reflections | 27600 | ||
| <I/σ(I)> | 5.7 | ||
| Completeness [%] | 97.6 | 98.2 | 96.6 |
| Redundancy | 2.1 | 2.1 | 2.1 |
| CC(1/2) | 0.997 | 0.461 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 30% PEG 4000, 100 mM PIPES pH 6, and 1M NaCl |
