5TQA

Crystal Structure of DH270.6 (unliganded) from the DH270 Broadly Neutralizing N332-Glycan Dependent Lineage

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97925
Spacegroup name	P 1 21 1
Unit cell lengths	67.066, 73.718, 112.714
Unit cell angles	90.00, 107.18, 90.00

Refinement procedure

Resolution	48.360 - 2.723
R-factor	0.1884
Rwork	0.185
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tpp
RMSD bond length	0.004
RMSD bond angle	0.912
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.360	50.000	2.780
High resolution limit [Å]	2.730	7.400	2.730
Rmerge	0.112	0.030	0.642
Rmeas	0.150	0.040	0.859
Rpim	0.099	0.027	0.565
Total number of observations	57894
Number of reflections	27600
<I/σ(I)>	5.7
Completeness [%]	97.6	98.2	96.6
Redundancy	2.1	2.1	2.1
CC(1/2)		0.997	0.461

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	30% PEG 4000, 100 mM PIPES pH 6, and 1M NaCl