5TQ1

Phospholipase C gamma-1 C-terminal SH2 domain bound to a phosphopeptide derived from the insulin receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	34.010, 55.140, 58.665
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.332 - 1.485
R-factor	0.1736
Rwork	0.172
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k44
RMSD bond length	0.004
RMSD bond angle	0.834
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.665	58.665	1.560
High resolution limit [Å]	1.485	4.690	1.485
Rmerge		0.041	0.223
Rmeas	0.064
Rpim	0.024
Total number of observations	104032
Number of reflections	18758
<I/σ(I)>	16.5	14.6	2.2
Completeness [%]	99.5	98.7	97.5
Redundancy	5.5	7.1	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	277	0.1M Tris (pH 9.0), 30% PEG MME 2000