5TP1
The structure of the C-terminus of virulence protein IncE from Chlamydia trachomatis bound to Mus musculus SNX5-PX domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.730, 110.100, 86.130 |
| Unit cell angles | 90.00, 90.34, 90.00 |
Refinement procedure
| Resolution | 67.838 - 2.310 |
| R-factor | 0.2058 |
| Rwork | 0.203 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hpc |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.640 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.840 | 2.430 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.170 | 1.282 |
| Number of reflections | 29845 | |
| <I/σ(I)> | 6.5 | 1.3 |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.987 | 0.332 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | SNX5/IncE in 20 mM Hepes pH 7.4, 100 mM NaCl, 1 mM TCEP was mixed with an equal volume of 26% (w/v) PEG 1500 |
