5TOU

STRUCTURE OF C-PHYCOCYANIN FROM ARCTIC PSEUDANABAENA SP. LW0831

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	93
Detector technology	CCD
Collection date	2012-05-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	67.583, 175.718, 194.823
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.930 - 2.040
R-factor	0.173
Rwork	0.172
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ha7
RMSD bond length	0.018
RMSD bond angle	1.769
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MLPHARE
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	2.040	2.040
Number of reflections	142799
<I/σ(I)>	32.04
Completeness [%]	96.7	92.1
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	0.1M SODIUM ACETATE TRIHYDRATE (PH 5.0) AND 18%(W/V) PEG4000 , VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277K