5TON

Crystal structure of AAT H143L mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-07-01
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	55.290, 124.010, 129.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.337 - 1.400
R-factor	0.1851
Rwork	0.184
R-free	0.19820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ajr
RMSD bond length	0.006
RMSD bond angle	0.899
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	55.340
High resolution limit [Å]	1.400
Number of reflections	174668
<I/σ(I)>	16.55
Completeness [%]	100.0
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	277.15	40mM NaoAc, 2mM PLP, 8% PEG 6,000