5TOI

Crystal Structure of the Marburg Virus VP35 Oligomerization Domain P4222

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-26
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 42 2 2
Unit cell lengths	46.490, 46.490, 310.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.420 - 2.190
R-factor	0.315
Rwork	0.312
R-free	0.34200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5toh
RMSD bond length	0.009
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.490	2.310
High resolution limit [Å]	2.190	2.190
Rmerge	0.492	0.649
Number of reflections	6946
<I/σ(I)>	12.1	0.9
Completeness [%]	34.7	1
Redundancy	11	3
CC(1/2)	0.993	0.418

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.5	0.2 M K/Na tartrate and 40% 2-methyl-2,4-pentanediol (MPD)