5TOH
Crystal Structure of the Marburg Virus VP35 Oligomerization Domain I2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 75.690, 35.000, 105.240 |
| Unit cell angles | 90.00, 105.28, 90.00 |
Refinement procedure
| Resolution | 53.020 - 2.010 |
| R-factor | 0.241 |
| Rwork | 0.239 |
| R-free | 0.26600 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MR-Rosetta |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.020 | 2.120 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.139 | 1.009 |
| Number of reflections | 7658 | |
| <I/σ(I)> | 8.3 | 1 |
| Completeness [%] | 42.1 | 2.4 |
| Redundancy | 2.2 | 2.1 |
| CC(1/2) | 0.980 | 0.783 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295.5 | 100 mM Sodium cacodylate pH 6.5, 100 mM Mg-acetate and 18% 2-methyl-2,4-pentanediol (MPD), cryo-protected by addition of 25% ethylene glycol |
