5TOF
Room temperature structure of ubiquitin variant u7ub25
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 2014-12-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.954 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 42.720, 42.720, 54.880 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.040 - 1.120 |
R-factor | 0.1557 |
Rwork | 0.154 |
R-free | 0.17510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
RMSD bond length | 0.016 |
RMSD bond angle | 1.569 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX (1.11_2556) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 22.040 | 22.040 | 0.990 |
High resolution limit [Å] | 0.960 | 4.290 | 0.960 |
Rmerge | 0.058 | 0.039 | 1.245 |
Number of reflections | 32428 | ||
<I/σ(I)> | 13.43 | 25.87 | 0.42 |
Completeness [%] | 90.2 | 53.9 | 39.5 |
Redundancy | 5.8 | ||
CC(1/2) | 0.998 | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 1ul protein solution at 20 mg/ml (20 mM Tris pH 8.0, 150 mM NaCl), 1ul crystallization solution (0.1 M citric acid pH 4.6, 2.6 M ammonium sulfate) |