5TO2
Crystal structure of streptavidin with one wild type subunit and three mutated subunits (N23A/S27D/S45A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.357, 56.738, 57.435 |
| Unit cell angles | 87.54, 85.89, 67.99 |
Refinement procedure
| Resolution | 50.000 - 1.650 |
| R-factor | 0.20575 |
| Rwork | 0.204 |
| R-free | 0.23365 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.444 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.068 | 0.458 |
| Number of reflections | 62294 | |
| <I/σ(I)> | 9.8 | 2.5 |
| Completeness [%] | 94.8 | 93.6 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris pH 8.3, 0.25 M MgCl2, 32% PEG4K |
