5TME
Crystal structure of Os79 from O. sativa in complex with UDP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-05 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | .979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.067, 83.306, 98.249 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.170 - 1.780 |
| R-factor | 0.18822 |
| Rwork | 0.186 |
| R-free | 0.22840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.093 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.780 |
| Rmerge | 0.053 |
| Number of reflections | 47323 |
| <I/σ(I)> | 47.3 |
| Completeness [%] | 99.9 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 277 | 50 mM HEPES pH 7.6, 10 mM glycine, 13% MEPEG 5000, 4% ethylene glycol |
