5TMD

Crystal structure of Os79 from O. sativa in complex with U2F and trichothecene.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	59.441, 83.155, 99.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.500 - 2.190
R-factor	0.16242
Rwork	0.160
R-free	0.21335
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.948
Data scaling software	HKL-3000
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.500
High resolution limit [Å]	2.190
Number of reflections	25837
<I/σ(I)>	19.1
Completeness [%]	99.6
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	100 mM MES pH 6.5, 200 mM glycine, 23% MEPEG 2000.