5TLK
COMPLEX BETWEEN HUMAN CD27 AND FAB FRAGMENTS OF ANTIBODIES M2177 AND H2191
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 139.710, 52.960, 140.600 |
| Unit cell angles | 90.00, 109.52, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.21422 |
| Rwork | 0.213 |
| R-free | 0.26318 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tl5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.250 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.062 | 0.487 |
| Number of reflections | 53961 | |
| <I/σ(I)> | 14.2 | 2.6 |
| Completeness [%] | 98.4 | 88.3 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M TRIS PH 8.5, 22% PEG 3350 |
