5TLJ
COMPLEX BETWEEN HUMAN CD27 AND FAB FRAGMENTS OF ANTIBODIES M2177 AND M2191
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-30 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 141.080, 52.960, 143.410 |
| Unit cell angles | 90.00, 112.19, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.28688 |
| Rwork | 0.283 |
| R-free | 0.35649 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tl5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.308 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.158 | 0.489 |
| Number of reflections | 25023 | |
| <I/σ(I)> | 9.6 | 2.7 |
| Completeness [%] | 97.8 | 85.5 |
| Redundancy | 4.4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M TRIS PH 8.5, 24% PEG 3350, 0.2 M AMMONIUM CHLORIDE |
