5TKZ

MEC-8 N-terminal RRM bound to tandem GCAC ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.873
Spacegroup name	P 32 2 1
Unit cell lengths	52.188, 52.188, 156.534
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.736 - 1.529
R-factor	0.2347
Rwork	0.233
R-free	0.27110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3md1
RMSD bond length	0.009
RMSD bond angle	1.177
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.740	1.584
High resolution limit [Å]	1.529	1.529
Rmerge	0.034	0.359
Number of reflections	38386
<I/σ(I)>	8.03	2.26
Completeness [%]	100.0	100
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 4000 10% (v/v), isopropanol 10%