5TIZ
Schistosoma japonicum (Blood Fluke) Sulfotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 57.308, 57.308, 181.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.648 - 2.870 |
| R-factor | 0.2297 |
| Rwork | 0.225 |
| R-free | 0.27630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mua |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.848 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.650 | 3.030 |
| High resolution limit [Å] | 2.870 | 2.870 |
| Rmerge | 0.667 | |
| Number of reflections | 7110 | |
| <I/σ(I)> | 7.7 | 2 |
| Completeness [%] | 96.9 | 98.8 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20%(v/v) PEG 8000, 3%(v/v) MPD, 0.1M Imidazole/Hydrochloric acid pH 6.5 |
