5THE
Crystal structure of the C-terminal lobe of a budding yeast Argonaute
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2014-10-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 119.609, 85.565, 127.864 |
| Unit cell angles | 90.00, 89.81, 90.00 |
Refinement procedure
| Resolution | 47.125 - 2.097 |
| R-factor | 0.1582 |
| Rwork | 0.158 |
| R-free | 0.20580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f1n |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.914 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1-2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.020 | 2.140 |
| High resolution limit [Å] | 2.097 | 2.100 |
| Number of reflections | 150631 | |
| <I/σ(I)> | 27.19 | 4.18 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | Sodium citrate 100 mM, pH 5.5 PEG4000 18% |
