5THA

Gemin5 WD40 repeats in complex with a guanosyl moiety

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-01-09
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	58.198, 125.253, 60.889
Unit cell angles	90.00, 117.10, 90.00

Refinement procedure

Resolution	32.510 - 1.800
R-factor	0.1687
Rwork	0.167
R-free	0.20040
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.015
RMSD bond angle	1.491
Data reduction software	XDS
Data scaling software	Aimless (0.5.27)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.510	32.510	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.053	0.016	0.596
Rmeas	0.062	0.019	0.696
Rpim	0.032	0.010	0.358
Total number of observations	260153	2119	14208
Number of reflections	69342
<I/σ(I)>	17.9	64	2.2
Completeness [%]	96.8	97.5	91.5
Redundancy	3.8	3.6	3.6
CC(1/2)	0.999	0.999	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Na-Citrate, pH 5.5, 0.2 M Ammonium Acetate, and 18% PEG4K with 1 mM GpppG