5TH0

Crystal structure of H10 hemagglutinin mutant (K158aA-Q226L-G228S) from Jiangxi-Donghu (2013) H10N8 influenza virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	64.493, 255.142, 70.285
Unit cell angles	90.00, 111.78, 90.00

Refinement procedure

Resolution	48.967 - 2.250
R-factor	0.2153
Rwork	0.213
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tgo
RMSD bond length	0.012
RMSD bond angle	2.160
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.290
High resolution limit [Å]	2.250	2.250
Number of reflections	95403
<I/σ(I)>	12.1	3.2
Completeness [%]	94.8
Redundancy	3	3
CC(1/2)		0.354

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	0.2 M NaCl, 10% (w/v) PEG 8000, 0.1M CHES pH 9.5