5TGX
Restriction/modification system-Type II R-SwaI complexed with partially cleaved DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-13 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.856, 57.058, 112.785 |
| Unit cell angles | 90.00, 108.22, 90.00 |
Refinement procedure
| Resolution | 49.000 - 2.300 |
| R-factor | 0.19992 |
| Rwork | 0.198 |
| R-free | 0.24197 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.928 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.370 |
| High resolution limit [Å] | 2.100 | 2.290 |
| Rmerge | 0.127 | 0.851 |
| Number of reflections | 53972 | |
| <I/σ(I)> | 12.5 | 1.1 |
| Completeness [%] | 96.1 | 79.9 |
| Redundancy | 6.4 | 4.1 |
| CC(1/2) | 0.613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 24 - 28% PEG1000, 100 mM TrisHCl, 5 mM CaCl2, |
