5TGV
Crystal structure of H10 hemagglutinin mutant (K158aA-D193T-Q226L-G228S) from Jiangxi-Donghu (2013) H10N8 influenza virus in complex with 3'-SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.512, 253.173, 69.964 |
| Unit cell angles | 90.00, 111.97, 90.00 |
Refinement procedure
| Resolution | 35.715 - 2.974 |
| R-factor | 0.191 |
| Rwork | 0.187 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tgu |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.498 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 38737 | |
| <I/σ(I)> | 5.93 | 1.06 |
| Completeness [%] | 93.3 | |
| Redundancy | 2.6 | 2.6 |
| CC(1/2) | 0.499 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 0.2 M NaCl, 10% (w/v) PEG 8000, 0.1M CHES pH 9.5 |
