5TGU
Crystal structure of H10 hemagglutinin mutant (K158aA-D193T-Q226L-G228S) from Jiangxi-Donghu (2013) H10N8 influenza virus in complex with 6'-SLNLN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.111, 253.873, 70.199 |
Unit cell angles | 90.00, 111.80, 90.00 |
Refinement procedure
Resolution | 38.630 - 2.350 |
R-factor | 0.1966 |
Rwork | 0.194 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tgo |
RMSD bond length | 0.012 |
RMSD bond angle | 2.330 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 85707 | |
<I/σ(I)> | 18.3 | 2.5 |
Completeness [%] | 98.3 | |
Redundancy | 3.1 | 3.1 |
CC(1/2) | 0.821 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 0.2 M NaCl, 10% (w/v) PEG 8000, 0.1M CHES pH 9.5 |