5TGQ
Restriction-modification system Type II R.SwaI, DNA free
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-05 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 0.92024 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 48.386, 65.225, 67.567 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 1.880 |
| R-factor | 0.20178 |
| Rwork | 0.198 |
| R-free | 0.27915 |
| Structure solution method | MIR |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.185 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.065 | 1.048 |
| Number of reflections | 16745 | |
| <I/σ(I)> | 31.6 | 1.13 |
| Completeness [%] | 71.0 | 73 |
| Redundancy | 12.5 | 6.4 |
| CC(1/2) | 0.656 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 16-20% PEG1000 (w/v) 100 mM TrisHCl, 20 - 40 mM Calcium Acetate, 150 mM NaBr |
