5TGN

Crystal structure of protein Sthe_2403 from Sphaerobacter thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-05
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97929
Spacegroup name	C 1 2 1
Unit cell lengths	90.178, 66.907, 90.635
Unit cell angles	90.00, 101.41, 90.00

Refinement procedure

Resolution	28.628 - 2.001
R-factor	0.163
Rwork	0.161
R-free	0.20490
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	0.940
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((dev_2386))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.096	0.960
Number of reflections	35690
<I/σ(I)>	26.88	2.75
Completeness [%]	100.0	100
Redundancy	11.3	11.3
CC(1/2)		0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	0.1 M Bis-Tris propane:NaOH, pH 7, 0.6 M K/Na tartrate, cryo 25% glycerol