5TGJ
Structure of the SNX5 PX domain in complex with chlamydial protein IncE in space group I2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 58.440, 80.320, 94.722 |
| Unit cell angles | 90.00, 97.94, 90.00 |
Refinement procedure
| Resolution | 31.900 - 2.600 |
| R-factor | 0.2035 |
| Rwork | 0.199 |
| R-free | 0.24260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.152 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.900 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.153 | 0.659 |
| Number of reflections | 13432 | |
| <I/σ(I)> | 39.6 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.986 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 295 | 0.1 M NaCl, 0.1 M MgCl2, 0.1 M Nacitrate (pH 3.5), 12 % PEG 4000 |
