5TGH

Structure of the SNX5 PX domain in complex with chlamydial protein IncE in space group P32

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95370
Spacegroup name	P 32
Unit cell lengths	100.640, 100.640, 71.650
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.179 - 2.800
R-factor	0.2378
Rwork	0.236
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tgi
RMSD bond length	0.015
RMSD bond angle	1.280
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.300	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.101	0.713
Number of reflections	20001	2923
<I/σ(I)>	11.7	2.3
Completeness [%]	100.0	100
Redundancy	5.8	5.8
CC(1/2)	0.997	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	295	1.26 M (NH4)2SO4, acetate (pH 4.5), 0.2 M NaCl