5TGA
Crystal structure of the S.cerevisiae 80S ribosome in complex with the A-site bound aminoacyl-tRNA analog ACCA-Pro
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 277 |
| Detector technology | PIXEL |
| Collection date | 2014-11-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 435.450, 288.140, 304.160 |
| Unit cell angles | 90.00, 99.11, 90.00 |
Refinement procedure
| Resolution | 149.313 - 3.300 |
| R-factor | 0.2125 |
| Rwork | 0.211 |
| R-free | 0.26490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.668 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (1.10.1_2155) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 200.000 | 3.400 | |
| High resolution limit [Å] | 3.300 | 125.000 | 3.300 |
| Rmerge | 0.333 | 0.311 | 1.485 |
| Number of reflections | 1026480 | ||
| <I/σ(I)> | 6.51 | 8.9 | 0.97 |
| Completeness [%] | 92.7 | 37 | 91.7 |
| Redundancy | 6 | ||
| CC(1/2) | 0.989 | 0.952 | 0.319 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG20000 |
