5TG9

Crystal structure of H15 hemagglutinin from A/shearwater/WA/2576/1979 H15N9 influenza virus in complex with 3'-SLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 3
Unit cell lengths	116.677, 116.677, 129.108
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.283 - 2.749
R-factor	0.2247
Rwork	0.222
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tg8
RMSD bond length	0.014
RMSD bond angle	1.899
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.750	2.750
Number of reflections	79813
<I/σ(I)>	10.8	1.7
Completeness [%]	97.4
Redundancy	2.7	2.6
CC(1/2)		0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	20% (w/v) PEG 3500, 0.2M potassium nitrate pH 6.9.