5TFS

Structure of chimeric 02-K Fab, a VRC01-like germline antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	47.525, 81.295, 121.719
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.270 - 2.319
R-factor	0.2027
Rwork	0.201
R-free	0.22790
RMSD bond length	0.003
RMSD bond angle	0.623
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.270
High resolution limit [Å]	2.319
Number of reflections	21164
<I/σ(I)>	16.5
Completeness [%]	99.7
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	30 % PEG 8000, 15 % isopropanol, 0.1 M Hepes pH 7.5, 0.2 M Ammonium Sulfate