5TFM
Crystal Structure of Human Cadherin-23 EC6-8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 93.407, 93.407, 122.386 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 80.890 - 2.920 |
| R-factor | 0.20006 |
| Rwork | 0.198 |
| R-free | 0.24635 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tfl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.428 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.890 | 2.970 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Rmerge | 0.079 | 0.592 |
| Number of reflections | 13518 | |
| <I/σ(I)> | 17.08 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 277 | 0.1 M MES pH 6.8, 1.2 M NaCl |
