5TFK
Crystal Structure of Mouse Cadherin-23 EC19-21 WT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.408, 66.932, 112.815 |
| Unit cell angles | 90.00, 98.72, 90.00 |
Refinement procedure
| Resolution | 111.510 - 2.800 |
| R-factor | 0.20318 |
| Rwork | 0.201 |
| R-free | 0.24386 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i8d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.546 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.510 | 2.860 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.065 | 0.504 |
| Number of reflections | 14163 | |
| <I/σ(I)> | 17.3 | 2 |
| Completeness [%] | 97.6 | 85.4 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 277 | 0.1 M Sodium Cacodylate pH 6.2, 0.075 M Calcium Acetate, 28% MPD |
