5TF3
Crystal Structure of Protein of Unknown Function YPO2564 from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.094, 75.094, 73.772 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.462 - 2.001 |
| R-factor | 0.1996 |
| Rwork | 0.198 |
| R-free | 0.21940 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.821 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (dev_2411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.104 | 0.606 |
| Number of reflections | 16095 | |
| <I/σ(I)> | 19.3 | 1.4 |
| Completeness [%] | 96.7 | 78.7 |
| Redundancy | 4.2 | 2.2 |
| CC(1/2) | 0.577 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1 M Hepes pH 7.5, 20%(w/v) PEG8000 |
