5TF0
Crystal Structure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.570, 88.447, 107.282 |
| Unit cell angles | 90.00, 97.89, 90.00 |
Refinement procedure
| Resolution | 39.169 - 2.202 |
| R-factor | 0.2174 |
| Rwork | 0.215 |
| R-free | 0.26420 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.889 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((dev_2411: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.475 | |
| Number of reflections | 72619 | |
| <I/σ(I)> | 11.6 | 2.2 |
| Completeness [%] | 96.4 | 72.3 |
| Redundancy | 4.2 | 2.2 |
| CC(1/2) | 0.667 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 289 | 0.2 M potassium nitrate pH 6.9, 20 5(w/v) PEG3350 |
