5TEB

Crystal Structure of the TIR domain from the Arabidopsis Thaliana disease resistance protein RPP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	81.890, 84.330, 122.750
Unit cell angles	90.00, 90.10, 90.00

Refinement procedure

Resolution	40.945 - 2.796
R-factor	0.2203
Rwork	0.218
R-free	0.26850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.988
Phasing software	PHENIX (1.11rc2_2531)
Refinement software	PHENIX (1.11rc2_2531)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.945	2.660
High resolution limit [Å]	2.550	2.580
Number of reflections	41428
<I/σ(I)>	14.5	1.3
Completeness [%]	99.6	95.6
Redundancy	7.6	6.9
CC(1/2)		0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	15% PEG 6000, 0.2 M citrate pH 5.5