5TE5
Crystal structure of Bos taurus opsin regenerated with 6-carbon ring retinal chromophore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-31 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 81.725, 81.725, 200.012 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.530 - 4.010 |
| R-factor | 0.31633 |
| Rwork | 0.314 |
| R-free | 0.35719 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g87 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.229 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 4.010 | |
| Rmerge | 0.243 | 5.625 |
| Number of reflections | 6944 | |
| <I/σ(I)> | 6.33 | |
| Completeness [%] | 99.8 | |
| Redundancy | 10.6 | |
| CC(1/2) | 0.999 | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2.8-3.4 M ammonium sulfate in 0.05-0.1 M MES, pH 6.1-6.6, or 0.05-0.1 M NaAcO buffer, pH 5.2-5.6 |
