5TDR

Set3 PHD finger in complex with histone H3K4me2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-03-02
Detector	NOIR-1
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	25.931, 43.720, 84.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.850 - 1.420
R-factor	0.1552
Rwork	0.153
R-free	0.19980
RMSD bond length	0.005
RMSD bond angle	0.893
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.860
High resolution limit [Å]	1.420
Number of reflections	18587
<I/σ(I)>	7.5
Completeness [%]	98.4
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	273	20% PEG3350, 0.2 M sodium citrate