5TDQ

Crystal Structure of the GOLD domain of ACBD3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-18
Detector	RAYONIX MX-300
Wavelength(s)	0.9797
Spacegroup name	P 31 2 1
Unit cell lengths	55.510, 55.510, 140.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.717 - 2.493
R-factor	0.2398
Rwork	0.238
R-free	0.26170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tlg
RMSD bond length	0.009
RMSD bond angle	1.151
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.720
High resolution limit [Å]	2.493
Rmerge	0.059
Number of reflections	5907
<I/σ(I)>	8.6
Completeness [%]	62.9
Redundancy	4.5
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	13% PEG 4000, 200 mM Ammonium Sulfate, 100 mM HEPES, 10% 2-propanol, 10% glycerol