5TDJ
RNA decamer duplex with four 2'-5'-linkages
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 27.538, 94.455, 21.283 |
| Unit cell angles | 90.00, 111.19, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.500 |
| R-factor | 0.15385 |
| Rwork | 0.152 |
| R-free | 0.17033 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ms9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.962 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.064 | 0.554 |
| Number of reflections | 7870 | |
| <I/σ(I)> | 18.3 | 1.8 |
| Completeness [%] | 96.7 | 88.7 |
| Redundancy | 3.9 | 3 |
| CC(1/2) | 0.649 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10% MPD (2-methyl-2,4-pentanediol), 40 mM sodium cacodylate pH 7.0, 12 mM spermine tetrahydrochloride, 40mM LiCl, 20 mM MgCl2 and 80 mM SrCl2 |
