5TDD
Human UBR-box from UBR2 in complex with HIFS peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.6362 |
| Spacegroup name | P 41 |
| Unit cell lengths | 29.313, 29.313, 74.151 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.727 - 1.550 |
| R-factor | 0.1106 |
| Rwork | 0.106 |
| R-free | 0.15210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ny3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.963 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.727 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.558 | |
| Number of reflections | 9083 | |
| <I/σ(I)> | 32.1 | 4.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.4 | 5.7 |
| CC(1/2) | 0.851 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES pH 6.5, 12% PEG 20000 |
