5TD7

Crystal structure of histone deacetylase 10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-30
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.28211
Spacegroup name	P 31 2 1
Unit cell lengths	80.449, 80.449, 243.037
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.506 - 2.850
R-factor	0.1839
Rwork	0.182
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eei
RMSD bond length	0.004
RMSD bond angle	0.713
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.850	2.850
Rmerge	0.119	0.930
Rpim	0.070	0.554
Number of reflections	22191	3182
<I/σ(I)>	12.9	2.2
Completeness [%]	99.9	100
Redundancy	7.1	7.3
CC(1/2)	0.997	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M KH2PO4, 20% PEG 3350