5TBJ
Crystal structure of mouse CARM1 in complex with inhibitor LH1452
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.844, 99.200, 207.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.320 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.23730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ih3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.904 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1980) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.970 | 2.380 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.230 | 1.796 |
| Number of reflections | 66284 | |
| <I/σ(I)> | 4.6 | 0.9 |
| Completeness [%] | 98.6 | 87.5 |
| Redundancy | 4.4 | 4.2 |
| CC(1/2) | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Tris-HCl pH 8.0 100 mM PEG 2000 MME 19% NaCl 100 mM |
