5TAB

Crystal Structure of the PHD Finger of PHF20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	46.739, 46.739, 50.928
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.688 - 1.250
R-factor	0.1206
Rwork	0.118
R-free	0.14350
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.472
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.690
High resolution limit [Å]	1.250
Number of reflections	33909
<I/σ(I)>	7.4
Completeness [%]	99.7
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277.15	0.1 M bis-tris propanol pH 6.5, 2.55 M ammonium sulfate titrated with 0.006 ml per 1 ml 1 N HCl