5T93
Immunoglobulin light chain variable domain AL-T05
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2012-01-28 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5241 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.459, 42.118, 64.995 |
| Unit cell angles | 80.66, 86.24, 63.02 |
Refinement procedure
| Resolution | 34.426 - 1.900 |
| R-factor | 0.1721 |
| Rwork | 0.171 |
| R-free | 0.22270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q20 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.103 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.100 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 | 0.073 |
| Number of reflections | 24891 | |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 88.0 | 51.49 |
| Redundancy | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2 M sodium acetate 0.1 M sodium cacodylate 30% PEG 8000 |
