5T8V

Chaetomium thermophilum cohesin loader SCC2, C-terminal fragment

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97935
Spacegroup name	P 1 21 1
Unit cell lengths	66.733, 88.779, 160.296
Unit cell angles	90.00, 93.17, 90.00

Refinement procedure

Resolution	36.240 - 2.798
R-factor	0.2293
Rwork	0.228
R-free	0.26730
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.488
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MLPHARE
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.240	36.240	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.106	0.029	1.103
Rmeas	0.121
Rpim	0.057
Total number of observations	229634
Number of reflections	51321
<I/σ(I)>	6.7
Completeness [%]	99.8	99.1	97.9
Redundancy	4.5	4.4	3.8
CC(1/2)		0.999	0.448

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	9% (w/v) PEG 6,000, 150 mM sodium citrate pH 5.2, 5 mM TCEP, 150 mM NaCl, 20 mM Tris pH 7.5, 15% ethylene glycol.