5T8N
Crystal structure of Snf7 under 200 MPa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-26 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.826, 47.844, 45.185 |
| Unit cell angles | 90.00, 98.87, 90.00 |
Refinement procedure
| Resolution | 44.644 - 2.204 |
| R-factor | 0.2529 |
| Rwork | 0.242 |
| R-free | 0.34480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fd9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.018 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.361 | |
| Number of reflections | 6164 | |
| <I/σ(I)> | 19.1 | 1.5 |
| Completeness [%] | 95.7 | 90.5 |
| Redundancy | 3.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 6% PEG20000, 110mM NaCl, 70mM Mes pH5.5 |
