5T84

HIV-1 protease, unbound subtype B L63P construct

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-04-15
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 2 21 21
Unit cell lengths	46.290, 58.010, 85.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.292 - 1.650
R-factor	0.179
Rwork	0.177
R-free	0.20230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.023
Data reduction software	iMOSFLM (7.1.1)
Data scaling software	Aimless
Phasing software	PHASER (2.1)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	17.470
High resolution limit [Å]	1.650
Number of reflections	26492
<I/σ(I)>	11
Completeness [%]	93.2
Redundancy	7.41

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	sodium chloride